Bigelovii A Protects against Lipopolysaccharide-Induced Acute Lung Injury by Blocking NF-κB and CCAAT/Enhancer-Binding Protein δ Pathways

Figure 1

Effect of Bigelovii A on acute lung injury (ALI) induced by airway administration of LPS. ALI was induced by intratracheal administration of LPS. 18 h (a–d) or 60 h (e–h) later, BAL fluids were extracted, and the whole lungs were harvested. (a and e) ELISA was conducted to detect mouse albumin levels in BAL fluids, and the permeability index was expressed as the ratio of the albumin in the LPS-treated lungs versus that in the PBS-treated lungs of same type of mice. (b and f) MPO contents in whole lung tissues were determined. Total cell (c and g) and neutrophil (d and h) accumulation in BAL fluids were estimated. Results are reflected by means ± SEM [ for Vehicle plus PBS group (negative control) or Bigelovii A+PBS group, for LPS+Vehicle group (positive control), and for LPS+Bigelovii A group]. and indicated statistically significant difference, and 0.001, respectively.

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