Bigelovii A Protects against Lipopolysaccharide-Induced Acute Lung Injury by Blocking NF-κB and CCAAT/Enhancer-Binding Protein δ Pathways

Figure 8

Effect of Bigelovii A on NF-κB activation during LPS-induced inflammation in MH-S cells. (a) MH-S cells were pretreated with or without 10 μM of Bigelovii A for 1 h, followed by treatment with PBS or 100 ng/mL of LPS for 3 h, and total proteins were isolated. Western blot was performed by using antibodies against NF-κB p-p65 and GAPDH, respectively. (b) MH-S cells were transiently transfected with total of 0.5 μg indicated plasmids. 48 h after transfection, the cells were pretreated with or without 10 μM of Bigelovii A for 1 h, followed by treatment with PBS or 100 ng/mL of LPS. 4 h later, the cells were lysed, and luciferase activity was measured. Luminometer values were normalized for expression from a cotransfected pRL-TK gene and negative control sequentially. Results are reflected by means ± SEM (). indicated statistically significant difference, . Negative control: cells treated by Vehicle plus PBS. Positive control: cells receiving LPS plus Vehicle treatment.

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