Disruption of Tumor Necrosis Factor Receptor-Associated Factor 5 Exacerbates Murine Experimental Colitis via Regulating T Helper Cell-Mediated Inflammation

Figure 7

TRAF5 deficiency significantly enhances the activation of p65 in CD4⁺ T cells after the administration of DSS. (a) Representative immunofluorescence staining for p65. The p65-labeled cells show red fluorescence, and the nuclei show blue fluorescence (magnification 400x). (b) Quantification of p65 positive cells. (c) Representative immunofluorescence staining for CD4 and p65. The CD4-labeled cells show green fluorescence, p65-labeled cells show red fluorescence, and the nuclei show blue fluorescence (magnification 400x). (d) Quantification of p65⁺CD4⁺ cells. (e) Representative immunofluorescence staining for CK18 and p65. The CK18-labeled cells show green fluorescence, p65-labeled cells show red fluorescence, and the nuclei show blue fluorescence (magnification 400x). (f) Quantification of p65⁺CK18⁺ cells. The data are representative of 3 independent experiments (mean ± SD). per group. versus the DSS-treated WT group. NS: not significant versus the DSS-treated WT group.

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