PGC-1α-Dependent Mitochondrial Adaptation Is Necessary to Sustain IL-2-Induced Activities in Human NK Cells

Figure 4

Inhibition of ATP synthase with oligomycin decreased cytotoxicity in NK cells and IL-2-activated NK cells. Human NK cells were incubated in culture for 72 h in the presence of 50 IU/mL (control) or 2000 IU/mL to induce LAK activity. When indicated, cells were pretreated with 5 μM oligomycin for 30 min. Cytotoxic activity was measured in a 4 h ⁵¹Cr release assay using Daudi cells as target cells and a 1 : 1 effector : target ratio. Results are mean ± SE from at least 7 independent experiments (; ).