Caspase-11 Modulates Inflammation and Attenuates Toxoplasma gondii Pathogenesis

Figure 1

IL-1β levels are ablated in macrophages harvested from Casp11^−/− and Asc^−/− mice following ex vivo infection with Toxoplasma gondii. Bone marrow derived macrophages were primed with 100 ng/mL of LPS for 30 mins prior to stimulation with either Toxoplasma gondii Me49 (MOI = 1) or vehicle for 24 hrs. IL-1β levels in cell-free supernatants were quantified using ELISA. ; . Data shown are representative of 3 independent experiments utilizing 2 mice per genotype.