Effects of Secreted Mast Cell Mediators on Retinal Pigment Epithelial Cells: Focus on Mast Cell Tryptase

Figure 8

Cell migration in the scratch assay. The scratch assay assessed in vitro migration of RPE cells stimulated by tryptase (36 nM and 180 nM). A uniform wound was made in the cell monolayer in each plate using a 200 μl pipette tip and was observed after 24 h. To quantitatively evaluate wound healing, total cell migration was determined with NIH image. (a) Control, (b) tryptase (36 nM), and (c) tryptase (180 nM). Tryptase induced RPE cell migration.