Research Article
A Novel Role for Brain Natriuretic Peptide: Inhibition of IL-1 Secretion via Downregulation of NF-kB/Erk 1/2 and NALP3/ASC/Caspase-1 Activation in Human THP-1 Monocyte
Figure 2
BNP activates NPR-1 in THP-1 cells. (a) THP-1 cells were treated with 10−8 M BNP in absence or presence of 10 μg/mL LPS/5 mM ATP and cGMP intracellular levels were evaluated after 5 and 30 min treatment. (b) THP-1 cells were treated with 10−8 M BNP in absence or presence of 10 μg/mL LPS/5 mM ATP for 30 min. Cell lysates were immunoblotted for NPR-1. The blots were stripped of the bound Ab and reprobed with mouse anti--actin, to confirm equal loading. Western blots are representative of three separate experiments. Histograms indicate means ± SD of three separate experiments each one tested in triplicate. , , and versus untreated cells.
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