Research Article
A Novel Role for Brain Natriuretic Peptide: Inhibition of IL-1 Secretion via Downregulation of NF-kB/Erk 1/2 and NALP3/ASC/Caspase-1 Activation in Human THP-1 Monocyte
Figure 4
BNP is involved in the second mechanism controlling IL-1β production by inhibiting NALP3, ASC, and Caspase-1 cascade activation in THP-1 cells. THP-1 cells were treated with 10−8 M BNP in absence or presence of 10 μg/mL LPS/5 mM ATP for 30 min. Cell lysates were immunoblotted for NALP3 (a), ASC (b), or Caspase-1 (c). The blots were stripped of the bound Ab and reprobed with mouse anti--actin, to confirm equal loading. Western blots are representative of three separate experiments. Histograms indicate means ± SD of three separate experiments each one tested in triplicate. and versus untreated cells and and versus LPS/ATP treated cells.
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