Interleukin-6 from Adipose-Derived Stem Cells Promotes Tissue Repair by the Increase of Cell Proliferation and Hair Follicles in Ischemia/Reperfusion-Treated Skin Flaps

Figure 3

IL-6 in ADSC-CM promoted flap recovery through an increase of cell proliferation. (a) Levels of IL-6 expression in the cell lysates (CL) or in the conditioned medium (CM) from ADSCs and Hs68 fibroblasts were examined by Western blot. (b) The intensity of IL-6 expression in ADSCs was stronger than it was in Hs68 cells, as shown by immunocytochemical staining. . (c) The conditioned media were collected from ADSCs pretreated with or without a control immunoglobulin G (IgG), an IL-6-neutralizing antibody (anti-IL-6), an IL-6 siRNA transfection (si-IL-6), or a control scrambled oligonucleotide sequence (si-Scramble, si-Con) transfection. Representative images of the I/R-induced skin flaps in the presence or absence of CM, IgG, anti-IL-6, si-Con, or si-IL-6 treatment on postoperative day 5. (d) Photographs of I/R-induced skin flaps in the presence or absence of ADSC-CM, ADSC+IgG-CM (IgG), ADSC+anti-IL-6 antibody-CM (anti-IL-6), si-Scramble-ADSC-CM (si-Con), or si-IL-6-ADSC-CM (si-IL-6). The sections were stained with PCNA antibodies. . (e) Higher magnification of the area enclosed by the rectangle in (d) is shown. . (f) Statistical analysis of PCNA-positive cells under HPF. for each group. versus the I/R group; ^† versus the CM group; ^ǂ versus the IgG or the si-Scramble group.