Relationship between Helicobacter pylori Infection and Plasmacytoid and Myeloid Dendritic Cells in Peripheral Blood and Gastric Mucosa of Children

Figure 1

Fluorescence minus one controls for the quantification of dendritic cell subsets. Fluorescence minus one (FMO) controls were prepared without adding a particular fluorochrome-conjugated antibody, for instance fluorescence minus fluorescein (FITC), fluorescence minus allophycocyanin (APC), fluorescence minus tandem conjugate of phycoerytrin (PE), and the cyanine dye Cy7 (PE-Cy7), and fluorescence minus PE, respectively. The gating for each channel was defined to exclude nearly all backgrounds from the FMO controls on the 2D plots of HLA-DR versus individual fluorescence.