RID: Evaluation of the Possible Inhibiting Effect of the Proinflammatory Signaling Induced by TNF-α through NF-κβ and AP-1 in Two Cell Lines of Breast Cancer

Figure 10

Effect of supernatants of cell lines U373, MDA-MB-231, and MCF-7 stimulated with TNF-α on neovascularization in culture of human endothelial cells HMEC1: (a) addition of 10 μL of culture medium MCDB131 supplemented with 10% SFB and 1% Anti-Anti 100x reagent; (b) addition of 5 μL of VEGF reagent; (c) addition of 10 μL of SNs of the U373 cell line stimulated with TNF-α; (d) addition of 10 μL of SNs from the U373 cell line infected with RID Adenovirus but not stimulated with TNF-α; (e) addition of 10 μL of SNs from the U373 cell line stimulated with TNF-α and infected with the RID Adenovirus; (f) addition of 10 μL of SNs of the cell line MDA-MB-231 stimulated with TNF-α; (g) addition of 10 μL of SNs of the MDA-MB-231 cell line infected with RID Adenovirus, but not stimulated with TNF-α; (h) addition of 10 μL of SNs of the MDA-MB-231 cell line infected with the RID adenovirus and stimulated with TNF-α; (i) addition of 10 μL of SNs of the MCF-7 cell line stimulated with TNF-α; (j) addition of 10 μL of SNs of the MCF-7 cell line infected with RID Adenovirus, but not stimulated with TNF-α; (k) effect of adding 10 μL of SNs of the MCF-7 cell line infected with the RID Adenovirus and stimulated with TNF-α.

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