RID: Evaluation of the Possible Inhibiting Effect of the Proinflammatory Signaling Induced by TNF-α through NF-κβ and AP-1 in Two Cell Lines of Breast Cancer

Figure 3

Evaluation of the possible protective effect of the NF-κβ pathway of the adenoviral protein RID in the cell lines of human astrocytoma U373 and breast cancer MDA-MB-231 and MCF-7 stimulated with TNF-α. Lane A shows the U373 cell line without stimulation. The band of the IκBα protein is observed. Lane B shows the effect of TNF-α stimulation on the U373 cell line not infected with Adenovirus. Lanes C and D show the U373 cell line infected with Adenovirus Null and RID, respectively. Lane E shows the MDA-MB-231 cell line without stimulation. Lane F shows the effect of stimulation with TNF-α in this cell line not infected with Adenovirus. The IκBα protein is degraded. Lanes G and H show infection of MDA-MB-231 cells with Adenovirus Null and RID, respectively. Lane I shows the MCF-7 cell line without stimulation. Lane J shows the effect of TNF-α stimulation on the MCF-7 cell line not infected with Adenovirus. The IκBα protein is degraded. Lanes K and L show the MCF-7 cell line infected with Adenovirus Null and RID, respectively. GADPH: used as loading control.