Toll-Like Receptor-Mediated Cardiac Injury during Experimental Sepsis

Figure 4

Effects of LPS on murine HL-cells. Fluorescence intensity of troponin I expression of HL-1 cells (a). Troponin I elevation in supernatant fluids from HL-1 cells (b) and from human cardiomyocytes (c). mRNA expression of pyrogenic receptor (P2X7) in human cardiomyocytes (d). HL-1 cells were treated with 20 μg/ml LPS and human cardiomyocytes (iPS) with 10 μg/ml LPS for 6 h (black bars). Additional treatment included treatment with 20 μg/ml or 10 μg/ml LPS for 5 h, respectively, and for one further hour with either 1 mM ATP or 1 μM nigericin (grey bars). Control groups were incubated in a cell culture medium without any supplements for 6 h (white bars). Amount of cellular reactive oxygen species (ROS) (e). Fluorescence intensity of ryanodine receptor 1 (RyR1) (f). per group; . All values are expressed as .

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