Research Article
TMEM100 Regulates Neuropathic Pain by Reducing the Expression of Inflammatory Factors
Table 2
Primary antibodies and IgG controls used in this study.
| | Host | Supplier/catalog number | Dilution |
| | TMEM100 | Rabbit polyclonal | Millipore/ABN1721 | 1 : 100(IHC), 1 : 500(Wb) | | TMEM100 | Mouse monoclonal | Origene/TA500532 | 1 : 100(IHC), 1 : 500(Wb) | | GFAP | Mouse monoclonal | Elabscience/E-AB-22022 | 1 : 200(IHC), 1 : 1000(Wb) | | Iba1 | Rabbit polyclonal | Abcam/ab178846 | 1 : 100(IHC), 1 : 1000(Wb) | | IL-1β | Rabbit polyclonal | Affinity/AF5103 | 1 : 1000(Wb) | | IL-6 | Rabbit polyclonal | Affinity/DF6087 | 1 : 1000(Wb) | | TNF-α | Rabbit polyclonal | Abcam/ab215188 | 1 : 1000(Wb) | | β-actin | Rabbit polyclonal | Elabscience/E-AB-20058 | 1 : 1000(Wb) | | Tubulin | Rabbit polyclonal | Elabscience/E-AB-20070 | 1 : 200(IHC) | | IgG control | Mouse | Elabscience/E-AB-1001 | 1 : 2000(Wb) | | IgG control | Rabbit | Elabscience/E-AB-1003 | 1 : 5000(Wb) | | IgG control | Mouse | Elabscience/E-AB-1015 | 1 : 100(IHC) | | IgG control | Rabbit | Elabscience/E-AB-1014 | 1 : 100(IHC) |
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TMEM100, transmembraneprotein100; GFA P, glial fibrillary acidic protein; Iba1, ionized calcium binding adaptor molecule 1; IL-1 β, interleukin-1 β; IL-6, interleukin-6; TNF- α, tumor necrosis factor α; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; Tubulin, β-tubulin; IgG, immunoglobulin G. |
