**1. Image Denoising** |

1.1 Find the rough position of each grid line; |

1.2 Remove the noise using median filtering locally. |

**2. Cell Extraction Individually** |

2.1 Guided boundary contour tracing; |

2.2 Cell Extraction through 4-neighborhood region growing. |

**3. Shape from Shading Using Linear Approximation** |

3.1 Image Irradiance Equation Deductio; |

3.2 3-D Shape Reconstructing. |

**4. Computing RBC shape surface feature** |

4.1 Compute mean curvature and Gaussian curvature using image convolution; |

4.2 Set a threshold for this two curvature. |

**5: Segmentation through multiscale surface fitting** |

More details is in Section 6: Experiment. |