| 1. Image Denoising |
| 1.1 Find the rough position of each grid line; |
| 1.2 Remove the noise using median filtering locally. |
| 2. Cell Extraction Individually |
| 2.1 Guided boundary contour tracing; |
| 2.2 Cell Extraction through 4-neighborhood region growing. |
| 3. Shape from Shading Using Linear Approximation |
| 3.1 Image Irradiance Equation Deductio; |
| 3.2 3-D Shape Reconstructing. |
| 4. Computing RBC shape surface feature |
| 4.1 Compute mean curvature and Gaussian curvature using image convolution; |
| 4.2 Set a threshold for this two curvature. |
| 5: Segmentation through multiscale surface fitting |
| More details is in Section 6: Experiment. |