Research Article

Estimating Network Kinetics of the MAPK/ERK Pathway Using Biochemical Data

Table 5

Western blotting time-course dataset for the MAPK pathway collected from the School for Cancer Studies in Glasgow University.

Time (min) Ras.GTP Total Ras Raf. Total Raf

0 35618 623520 300095 824192
20 240222 672847 1092111 850161
40 385132 989878 1005269 865119
60 212275 1169001 1570648 887820
120 718095 898817 810234 785545
180 559659 1332240 1142328 1105969
240 525533 1240655 867336 948456
360 555147 1049890 1028401 1141482

Time (min) MEK.p2 Total MEK ERK.p2 Total ERK

0 67914 772901 98683 2470824
20 662655 718812 472934 2430305
40 729792 748227 678992 2428199
60 539726 954104 536933 2071863
120 559206 889207 290295 2396161
180 487896 846376 594756 2529436
240 501463 1030196 295292 2038472
360 449943 856206 408216 1636536