The Vector Population Monitoring Tool (VPMT): High-Throughput DNA-Based Diagnostics for the Monitoring of Mosquito Vector Populations

Table 2

Approximate cost of each of the VPMT assays per mosquito specimen analysed. In each case costings were calculated in US$ by using retail prices from US websites. These prices do not include promotional or negotiated discounts or the cost of delivery. All primer/probe/PCR MasterMix costs are from ordering at the largest available scale. Cost calculations include all the plastics required to set up the reactions. For the cost of the kdr and Rdl assays, this is the combined cost of running two assays (i.e., A296G + A296S).

Assay	Cost in US$
An. gambiae species ID (2 plex assay)	0.75
An. gambiae species ID (3 plex assay)	1
An. funestus species ID	0.95
Plasmodium detection assay	0.75
Kdr assay	1.5
iAChE assay	0.75
Rdl assay	1.5