In Vivo Hemozoin Kinetics after Clearance of Plasmodium berghei Infection in Mice

Figure 6

Hemozoin quantification by colorimetric assay in the organs of malaria infected-cured mice. Hemozoin (Hz) deposition in livers and spleens of P. berghei NK65 infected-cured mice was also determined by a colorimetric assay (QuantiChrom Heme Assay Kit) and expressed in haem equivalents (normalized to protein content). Overall Hz content seemed to increase overtime, although only slightly so for the liver. Liver and spleen of naïve mice had negligible amounts of detected haem of 468 and 890 μM haem/protein (mg/mL), respectively, which is likely to be associated with the presence of erythrocytes in the tissues.