In Vivo Hemozoin Kinetics after Clearance of Plasmodium berghei Infection in Mice
Figure 7
Detection of hemozoin by flow cytometry in the cells from malaria infected-cured mice. Detection of hemozoin (Hz) in phagocytic cells obtained from P. berghei NK65 infected-cured mice at different times after treatment, based on depolarized Side-Scatter measurement. Single cell suspensions from liver, spleen, and bone marrow were labelled with anti-CD11b-FITC and anti-Gr1-PE (blood samples) or anti-CD11b-FITC and anti-F4/80-PE (tissue samples). Hz could be detected in phagocytic cells in all infected mice while uninfected mice were negative for depolarizing cells. High numbers of Hz-containing phagocytic cells could be detected at the first time point (7 days after the end of CQ treatment) in livers (c) and spleens (d) with median levels of 56.5% and 24.4%, respectively. However, there was a marked decrease at later time points which seemed to become stable between 90, and 196 days after the end of CQ treatment. In bone marrow (b) Hz-containing phagocytes were less frequent overtime (starting at median levels of 2%) and in whole-blood (a) were overall low (median of 1.7% on first time point).
