Increased Prevalence of Mutant Allele Pfdhps 437G and Pfdhfr Triple Mutation in Plasmodium falciparum Isolates from a Rural Area of Gabon, Three Years after the Change of Malaria Treatment Policy

Table 2

Prevalence of Pfdhfr/Pfdhps haplotypes.

Haplotypes: Pfdhfr/Pfdhps	2005		2008
		%		%
108N-59C/R-51I-437G (NC/RIG)	0/29	0	2/55	3.6	NS
108N-59R-51I-437A/G (NRIA/G)	1/29	3.4	0/55	0	NS
108N-59R-51I-A437 (NRIA)	15/29	51.7	16/55	29.9	0.04
108S-59R-51I-437G (SRIG)	0/29	0	1/55	1.8	NS
Quadruple mutation: 108N-59R-51I-437G	10/29	34.4	35/55	64.8	0.01

Parasites carrying mixed infections designated by two genotypes at the codon indicated above. NS: not significant. For the calculation of the haplotype frequencies, samples with both dhfr and dhps genes analysis have been selected.