Treatment with Anti-EGF Ab Ameliorates Experimental Autoimmune Encephalomyelitis via Induction of Neurogenesis and Oligodendrogenesis

Figure 4

Increased numbers of de novo oligodendrocytes in the SVZ in response to therapy with anti-EGF Ab. Immunohistochemical labeling of BrdU⁺O4⁺ cells in the SGZ and SVZ of IC-treated mice (a and d, resp.) and of anti-EGF Ab-treated mice (b and e, resp.) on day 18 after immunization. Quantification of %BrdU⁺O4⁺ cells in the SGZ and in the SVZ (c and f, correspondingly) revealed a nonsignificant trend for induction of BrdU⁺O4⁺ in the SGZ, along with a significant induction in BrdU⁺O4⁺ cells in the SVZ. Images were obtained using a confocal microscopy, coronal sections. Quantification was performed using ZEN 2011 software on 3 sections from each mouse (3 mice from each group, total ). DG, dentate gyrus, LV, lateral ventricle, SVZ, subventricular zone, and cc, corpus callosum. Scale bar: 10 m (magnification = ×63).

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