The mGlu2/3 Receptor Agonists LY354740 and LY379268 Differentially Regulate Restraint-Stress-Induced Expression of c-Fos in Rat Cerebral Cortex
Figure 4
(a) Restraint stress upregulated c-Fos in the PrL cortex. Pretreatment with the mGlu2/3 agonist LY354740 (10 and 30 mg/kg, IP) but not LY379268 (10 mg/kg, i.p.) reversed restraint-induced increase in c-Fos expression in the PrL cortex. ** indicates significantly different from vehicle + home cage; , ***; # indicates significantly different from vehicle + restraint stress; ##. One-way ANOVA with Newman-Keuls post hoc test. Each bar represents the mean (±SEM), -8. (b) Representative photomicrographs of the prelimbic cortex showing c-Fos-labeled neurons of rats treated either with vehicle (Veh), LY (LY354740 30 mg/kg), vehicle + stress (vehicle + restraint stress), or LY + stress (LY354740 30 mg/kg + restraint stress). Restraint stress increased the number of Fos-positive-labeled cells and pretreatment with LY354740 (30 mg/kg) reversed the effect. LY354740 alone did not enhance Fos immunoreactivity. Scale bar = 100 μm.
