Research Article

Effect of Prenatal Protein Malnutrition on Long-Term Potentiation and BDNF Protein Expression in the Rat Entorhinal Cortex after Neocortical and Hippocampal Tetanization

Table 2

Changes in BDNF expression (pg/mg wet tissue) in the left entorhinal cortex (EC) of 55–60-day-old normal and prenatally malnourished rats two hours after applying ipsilateral tetanizing stimulation to the occipital cortex (OC) or the CA1 hippocampal region, as compared to BDNF levels in the right EC. Values are means ± SEM. The number of samples in each group is shown in parentheses. BDNF concentrations in right EC samples after tetanizing the left OC or left CA1 did not significantly differ between them, and were therefore pooled. Comparisons of BDNF levels between normal and malnourished groups were made using unpaired Student’s 𝑟 2 = 0 . 9 1 0 6 -test, and 𝑃 < . 0 5 is the probability level for comparisons related to the nutritional condition (NS = not significant). Comparisons between basal BDNF levels (right EC) with those obtained after OC or CA1 tetanization (left EC) were made using nonparametric ANOVA (Kruskal-Wallis test) followed by Dunn’s multiple comparisons post-hoc test, and 𝑃 < . 0 5 is the probability level for comparisons between right and left EC samples (different superscripts indicate a significant difference, 𝑡 ; NS = not significant).

Pooled OC + CA1 tetanizationOC tetanizationCA1 tetanization 𝑡
(right EC)(left EC)(left EC)

Normal 𝑃 N C (8) 𝑃 T (4) 𝑃 < . 0 5 (4)<0.05
Malnourished 𝑃 T (8) 1 5 . 7 ± 3 . 5 a (4) 1 4 . 4 ± 2 . 6 a (4)NS
2 5 . 1 ± 2 . 2 b <0.05NS<0.001