Visual Deprivation Decreases Somatic GAD65 Puncta Number on Layer 2/3 Pyramidal Neurons in Mouse Visual Cortex
Figure 2
Visual experience bidirectionally changes the number of GAD65 puncta contacting the soma of pyramidal neurons in layer 2/3 of visual cortex without altering the size or intensity of GAD65 puncta. (a) Dark rearing from birth for 5 weeks (DR) significantly decreased the number of GAD65 puncta contacting neuronal soma compared to normal reared mice of equivalent age (NR). Exposing the dark reared mice to light for 3 days (LE) increased the number of GAD65 puncta close to NR levels. Asterisks indicate statistical significance of using a Fisher’s PLSD posthoc analysis following a one-way ANOVA. (b) The soma volume of YFP expression neurons in layer 2/3 used for measuring GAD65 puncta number. There was a statistically significant decrease in soma volume of DR mice compared to NR and LE groups. Asterisks: using a Fisher’s PLSD posthoc analysis following a one-way ANOVA. (c) Visual experience did not significantly alter the size of GAD65 puncta contacting the soma of layer 2/3 pyramidal cells. (d) There was no statistically significant difference in the intensity of GAD65 puncta contacting the soma of layer 2/3 pyramidal cells across the three groups.
