]>Visual Deprivation Decreases Somatic GAD65 Puncta Number on Layer 2/3 Pyramidal Neurons in Mouse Visual Cortex : Figure 3
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Figure 3: Two dimensional analysis of total GAD65 intensity and puncta density. (a) The total GAD65 staining intensity in a single focal plane was decreased in the DR group when compared to NR and LE (normalized total GAD65 intensity: NR = 100 ± 9.3%, 𝑛 = 2 0 sections from 4 mice; DR = 45 ± 6.8% of average NR, 𝑛 = 1 7 sections from 4 mice; LE = 82 ± 15.7% of average NR, 𝑛 = 1 8 sections from 4 mice; ANOVA: 𝐹 ( 2 , 5 2 ) = 7 . 3 4 1 , 𝑃 < . 0 1 ). Asterisks: 𝑃 < . 0 5 using Fisher’s PLSD posthoc analysis. (b) The measurement of GAD65 puncta per area decreased on average in the DR group, but did not show a significant reversal with a subsequent light exposure (GAD65 puncta number per 𝜇 m 2 : NR = 0.48 ± 0.08, 𝑛 = 2 0 sections from 4 mice; DR = 0.13 ± 0.04, 𝑛 = 1 7 sections from 4 mice; LE = 0.30 ± 0.06, 𝑛 = 1 8 sections from 4 mice; ANOVA: 𝐹 ( 2 , 5 2 ) = 6 . 0 3 0 , 𝑃 < . 0 1 ). Asterisks: 𝑃 < . 0 3 using Fisher’s PLSD posthoc test.