Research Article

Nestin Reporter Transgene Labels Multiple Central Nervous System Precursor Cells

Figure 5

Phenotypic identification of eGFP+ cells in the adult SVZ. (a) eGFP+ cells immunolabeled with antinestin antibody; SVZ, P84. Cells appear to have just gone through cytokinesis. Scale bar = 10 microns. (b) eGFP+ cell immunolabeled with antinestin antibody; SVZ, P84. Scale bar = 5 microns. (c) Long arrow shows bright, and short arrow dim, eGFP+ cells in the ventral ependymal layer of the lateral ventricle labeled with antinestin antibody. Scale bar = 10 microns. (d) eGFP+ cell (arrow) expressing GFAP immunoreactivity in the SVZ of a P28 mouse. Bright cell indicated with arrow has nucleus filled with eGFP and is surrounded by GFAP in extending processes. Other cells (arrowhead) are in GFAP-negative areas. (e) Some eGFP+ cells coexpress the transcription factor Dlx. Scale bar = 10 microns. (f) eGFP+ could also be Mash1+. Scale bar = 10 microns. (g) Some eGFP+ cells (arrowheads) coexpress doublecortin in the RMS, whereas others (arrow) do not. Scale bar = 50 microns. (h) eGFP+ cells in the SVZ (arrow) do not express the microglial marker CD11b+ microglia (arrowhead). Scale bar = 50 microns.
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