Nestin Reporter Transgene Labels Multiple Central Nervous System Precursor Cells

Figure 9

Parenchymal astrocytes do not express eGFP. (a) GFAP+ astrocytes are not constitutively labeled with eGFP transgene (P84 striatum). Scale bar = 20 microns. (b) Cortical lesions (les) were performed to determine if reactive astrocytes express eGFP. Coronal section adapted from Paxinos’ atlas (Paxinos and Franklin, 2001). (c) The number of eGFP+ cells in the SVZ increased 10 days after injury (^∗∗𝑃<.01, 𝑡-Test). (d) eGFP+ cells in the denervated striatum did not colabel with GFAP immunohistochemistry (reactive astrocytes 10 days after injury).

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