Table 1: Comparison of nestin-reporter mice.

Yamaguchi et al. [8]Aoki et al. [7]Kawaguchi et al. [9]Mignone et al. [12]Beech et al. [11] Our work

Transgenic strategySequence that drives transgene2nd Intron, nestin promoter2nd Intron, thymidine kinase minimal promoter (a)2nd Intron, hsp68 min promoter2nd Intron, 5.8 kb nestin promoter5.8 kb upstream to 5.4 kb downstream of nestin Gene (b)2nd Intron, thymidine kinase promoter
Reporter moleculeeGFP 𝛽 -galactosidaseeGFPeGFPCREBModified (stable) eGFP (c)

General featuresEndogenous nestin coexpressionn.dYesYes (d)Yesn.d. (e)Yes
Brdu+Yes (f)n.d. (g)Yes (h)YesYesYes

Embryonic expressionVentricular zoneYesYesYesYesn.d.Yes
Subventricular zoneYesn.d.YesYesn.d.Yes
Pallium versus subpalliumn.d.n.d.n.d.Non.d.Yes
3d ventricleYesYesYesYesn.d.Yes
Spinal cordYesYesYesn.d.n.d.n.d.
Postmitotic parencymaYesn.d.? (i)Non.d.Yes
Nonneural tissuesNoNon.d.Non.d.Yes

Postnasal and/or adult expression light microscopyNeurogenic regionsYesYesYesYesYes-subset (j)Yes
Dentate gyrus progenitorsYes?n.d.YesNoYes
Dentate gyrus neuronsYes?n.d.NoNoNo
SVZ B cellsYesNoYes (k)Yes? (l)Yes-subset
SVZ C cellsn.d.n.d.No (m)n.d.?Yes-subset
SVZ/RMS A cellsYesn.d.n.d.YesYes-subsetYes-subset
Increased in SVZ after injuryn.d.n.d.n.d.n.d.n.d.Yes
Ependymal cellsYesn.d.YesYes? (l)Yes-subset
OB
 GranuleYes?n.d.NoNoYes
 neurons
 PeriglomerularYes?n.d.YesYes-subsetYes
 neurons
 Accessory OBn.d.n.d.n.d.n.d.n.d.Yes
 neurons
Nonneurogenic regionsYes (n)Yesn.d.NoYes (o)Yes
Oligodendro- cytes?n.d.n.d.Non.d.Yes
Astrocytes?Non.d.n.d.n.d.No
Astrocytes after injuryn.d.No (p)n.d.n.d.n.d.No
Neurons?Yesn.d.Non.d.Yes-weak
Nonneural tissuen.d.n.d.n.d.n.d.n.d.Yes-hair follicles

Electron microscopySVZ B cellsn.d.n.d.n.d.n.d.n.d.Yes
SVZ C cellsn.d.n.d.n.d.n.d.n.d.Yes
SVZ A cellsn.d.n.d.n.d.n.d.n.d.Yes
Adult ependymal cellsn.d.n.d.n.d.n.d.n.d.Yes
Oligodendro- cytesn.d.n.d.n.d.n.d.n.d.Yes

Cell cultureNeurospheresn.d.n.d.Yes (q)Yesn.d.Yes (r)

n.d.: not determined. ?: not clear
(a) Line 3D6—includes mutation of SHP2 tyrosine phosphatase; (b) nestin-tTA mouse crossed with tetracycline regulated CREB used as readout; (c) Okada et al., 1999; (d) shown in vitro; (e) nestin expressed in the same general areas as transgene, cell coexpression not determined; (f) tested postnatally; (g) BrdU labeling index not changed, colabelling with transgene not done; (h) embryonically in vivo and in vitro; (i) parenchymal GFP+ cells visible in Figure 1(g); (j) SVZ but not dentate gyrus; (k) as determined by BrdU retention; (l) S 100b-labelling in Figure 4(a) looks ependymal not astrocytic; (m) determined by lack of BrdU pulse labelling; (n) glial morphology; (o) not labelled with BrdU; (p) not within 6 hrs post-MCAo; (q) embryonically, self-renewal and multipotency shown; (r) postnataly, self-renewal and multipotency shown.