Loss of Prestin Does Not Alter the Development of Auditory Cortical Dendritic Spines

Figure 5

Immunohistochemical analysis of excitatory synapses in wildtype and prestin KO animals. (a) Labeling of puncta positive for the excitatory marker PSD-95 in layer 2/3 of A1. (b) Density of PSD-95+ puncta did not differ significantly between WT (𝑛=3) and KO (𝑛=3) mice in L2/3 of A1 (𝑃>.05). (c) The size of PSD-95+ puncta is significantly greater in KO versus WT in L2/3 of A1 (𝑃=.015). (d) PSD-95+ puncta in layer 4 of A1. (e) Density of PSD-95+ puncta did not differ significantly between WT (𝑛=3) and KO (𝑛=3) mice in L4 of A1 (𝑃>.05). (f) The size of PSD-95+ puncta did not differ significantly between WT and KO mice in L4 of A1 (𝑃>.05). Scale bars: 10 μm.

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