Research Article
Deafferentation-Induced Redistribution of MMP-2, but Not of MMP-9, Depends on the Emergence of GAP-43 Positive Axons in the Adult Rat Cochlear Nucleus
Table 1
Experimental groups.
| | Group | Surgical procedures | POD following cochlear ablation | Number of brains | Immunohistochemical procedures |
| | 1 | — | — | 5 | GAP-43 ABC-DAB
MMP-2 ABC-DAB + flat-embedding + semithin sectioning
MMP-9 ABC-DAB + flat-embedding + semithin sectioning | | 2 | PBS injection into VNTB
→ recovery for 1–3 weeks | — | 2 | GAP-43 ABC-DAB
MMP-2 ABC-DAB + flat-embedding
MMP-9 ABC-DAB + flat-embedding | KA injection into VNTB
→ recovery for 1–3 weeks | — | 2 | GAP-43 ABC-DAB
MMP-2 ABC-DAB + flat-embedding
MMP-9 ABC-DAB + flat-embedding | | 3 | Cochlear ablation | POD1
POD3
POD7 | 4
3
5 | GAP-43 ABC-DAB
MMP-2 ABC-DAB + flat-embedding + semithin sectioning
MMP-9 ABC-DAB + flat-embedding + semithin sectioning | | 4 | KA injection into VNTB
→ recovery for 1-3 weeks
→ cochlear ablation | POD3
POD7 | 3 cL/2 iL
5 cL/3 iL | GAP-43 ABC-DAB
MMP-2 ABC-DAB + flat-embedding
MMP-9 ABC-DAB + flat-embedding |
|
|
cL: complete lesion of VNTB; DAB: diaminobenzidine staining; iL: incomplete lesion of VNTB; KA: kainic acid; PBS: phosphate buffered saline; POD: postoperative day.
|
