Identification and Characterization of Two Novel RNA Editing Sites in grin1b Transcripts of Embryonic Danio rerio

Figure 3

F3/R3 product. RT-PCR products (Invitrogen MMLV-RT, GoTaq Green Polymerase). Several reactions successfully produced robust bands at the predicted sizes: 200 and 209 nucleotides for F1/R1 and F2/R2, respectively, and 311 nucleotides for F3/R3. Since the product of primer pair F3/R3 (outlined in a red box) encompassed all 6 possible editing sites, this RT-PCR band product was extracted for sequencing (100 bp ladder, New England Biolabs, Ipswich, MA).