Studies of Olfactory System Neural Plasticity: The Contribution of the Unilateral Naris Occlusion Technique
Figure 1
Anatomical and histological effects of UNO. (a) Adult mouse that underwent left UNO as a neonate. Note the normal right naris and apparently unaffected morphology around the location of the occluded naris. (b) Bernhard von Gudden’s drawing of the brain from a young adult rabbit that had undergone UNO as a neonate ([4]; dorsal view reflected right/left to match other panels; occl: occluded). (c) Horizontal section through the olfactory bulbs of a young-adult mouse that had UNO as a neonate. Note all layers of the occluded bulb (left) are thinner than open-side bulb on the right (ONL: olfactory nerve layer; GL: glomerular layer; EPL: external plexiform layer; MCL: mitral cell layer; IPL: internal plexiform layer; GCL: granule cell layer; AOB: accessory olfactory bulb). (d) Histological sections through olfactory mucosa of young-adult mouse that had UNO as a neonate. Right column: open-side; left column: occluded side; top row: H&E stain; bottom row: OMP immunolabeling (arrows: mature OSN cell bodies) (layers: 1: sustentacular layer; 2: olfactory receptor cells; 3: basal cell layer; 4: lamina propria).
