Regulation of Ca²⁺/Calmodulin-Dependent Protein Kinase II Signaling within Hippocampal Glutamatergic Postsynapses during Flurazepam Withdrawal

Figure 2

Pre- and postsynaptic distribution of CaMKII and pCaMKII immunogold particles in CA1 asymmetric synapses of control and FZP-withdrawn rats. (a) Analysis of CaMKII postsynaptic labeling revealed a significant interaction between experimental group and binned distance (𝑃<0.05, F(6,48)=3.13, two-way repeated measures ANOVA) with significantly decreased CaMKII labeling 60 nm postsynaptic to the PSD membrane in FZP-withdrawn relative to control synapses (CON: 14.01±3.57 particles/μm, 𝑛=5; FZP: 5.32±1.45 particles/μm, 𝑛=5; 𝑃<0.01, Bonferroni’s posttest). In contrast, no significant interaction between treatment and binned distance was observed for presynaptic CaMKII (𝑃=0.34, F(6,48)=1.16, two-way repeated measures ANOVA). (b) There was no significant interaction between experimental group and binned distance for pre- (𝑃=0.96, F(6,38)=0.25) or postsynaptic pCaMKII labeling (𝑃=0.78, F(6,48)=0.53, two-way repeated measures ANOVA).

(a)

(b)