Research Article
Regulation of Ca2+/Calmodulin-Dependent Protein Kinase II Signaling within Hippocampal Glutamatergic Postsynapses during Flurazepam Withdrawal
Table 3
CaMKIIα and pCaMKII immunogold non-PSD synaptic labeling.
| | Presynaptic | Postsynaptic | | Active zone | Membrane | Intracellular | Perisynaptic membrane | Extrasynaptic membrane | Intracellular | | Labeled (%) ≥1 particle | Mean Number of particles | Labeled (%) ≥1 particle | Mean Number of particles | Labeled (%) ≥1 particle | Mean Number of particles | Labeled (%) ≥1 particle | Mean Number of particles | Labeled (%) ≥1 particle | Mean Number of particles | Labeled (%) ≥1 particle | Mean Number of particles |
| CaMKII | | | | | | | | | | | | | CON | 4.0 ± 1.3 | 0.04 ± 0.01 | 3.0 ± 1.5 | 0.04 ± 0.02 | 26.2 ± 7.6 | 0.54 ± 0.20 | 0.8 ± 0.5 | 0.02 ± 0.01 | 3.9 ± 1.4 | 0.07 ± 0.04 | 38.8 ± 9.9 | 0.86 ± 0.22 | FZP | 0.8 ± 0.5 | 0.008 ± 0.005 | 2.6 ± 1.3 | 0.03 ± 0.01 | 19.8 ± 3.0 | 0.39 ± 0.12 | 2.6 ± 1.6 | 0.03 ± 0.02 | 4.8 ± 2.6 | 0.06 ± 0.04 | 32.2 ± 5.7 | 0.59 ± 0.12 | value | 0.049
* | 0.047
* | 0.83 | 0.88 | 0.45 | 0.54 | 0.37 | 0.70 | 0.75 | 0.94 | 0.58 | 0.32 |
| pCaMKII | | | | | | | | | | | | | CON | 7.2 ± 2.3 | 0.09 ± 0.03 | 4.5 ± 2.5 | 0.10 ± 0.08 | 43.7 ± 9.7 | 1.29 ± 0.46 | 0.9 ± 0.9 | 0.01 ± 0.01 | 2.3 ± 1.1 | 0.02 ± 0.01 | 21.9 ± 10.5 | 0.62 ± 0.34 | FZP | 7.3 ± 2.2 | 0.08 ± 0.03 | 6.7 ± 3.0 | 0.09 ± 0.04 | 58.6 ± 7.6 | 1.67 ± 0.40 | 3.1 ± 2.2 | 0.04 ± 0.03 | 5.1 ± 2.8 | 0.07 ± 0.05 | 26.9 ± 7.8 | 0.49 ± 0.18 | value | 0.98 | 0.96 | 0.60 | 0.87 | 0.26 | 0.56 | 0.40 | 0.38 | 0.37 | 0.36 | 0.71 | 0.76 |
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, Student’s t-test. Values represent mean ± SEM.
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