Figure 2: CREB and pCREB levels during early postnatal development in mouse dLGN. (a) Representative western blots of CREB and pCREB protein levels measured in dLGN at P7, P14, and P21 using infrared imaging detection. Red represents CREB levels and green pCREB and GAPDH levels. Both CREB and pCREB levels decreased with age. ((b) and (c)) Quantification of immunoblots for CREB (c) and pCREB (d) using infrared imaging. The bars depict the relative averaged optical densities measured at P7, P14, and P21. CREB and pCREB levels were significantly higher at P7 than at P14 and P21 (LSD post-hoc tests, ). (d) Representative western blots of CREB and pCREB protein levels measured in dLGN at the same postnatal ages as in (a) using chemiluminescence detection. ((e) and (f)) Quantification of immunoblots for CREB (e) and pCREB (f) using chemiluminescence. Similar to infrared imaging, CREB and pCREB levels significantly decreased with age ((e) LSD post-hoc test and (f) Tamhane’s post-hoc test; ). Error bars represent ±SEM. Infrared imaging detection, blots; chemiluminescence, -4 blots; –8 dLGNs in each age group per blot for both techniques.