Figure 3: Modulation of CREB immunofluorescence in the dLGN of dark-reared mice. ((a)–(f)) Representative coronal sections of dLGN showing the labeling of total CREB ((a)–(c)) and pCREB ((d)–(f)), in NR ((a) and (d)), CDR ((b) and (e)), and DDR ((c) and (f)) mice. Corresponding high-magnification insets show that staining was prominent in somata. Py, pyramidal cell layer of hippocampus; Or, oriens layer of hippocampus; D, dorsal; L, lateral. Scale bars, 100 μm and 25 μm. ((g) and (h)) Cumulative histograms of cellular fluorescence intensity measured in somata in NR, CDR, and DDR for total CREB (g) and pCREB (h). Note that, for both total CREB and pCREB, CDR and DDR significantly shifted the distribution of fluorescence intensity toward larger values when compared to NR (the Mann-Whitney tests, for all comparisons). For CREB; NR cells, CDR , DDR ; for pCREB; NR cells, CDR , DDR ; from 2–6 dLGNs per group.