Maintenance of Synaptic Stability Requires Calcium-Independent Phospholipase A2 Activity
Figure 2
Inhibition of iPLA2γ induces Tau phosphorylation in area CA1 of hippocampus. Cultured hippocampal slices from P301L Tau transgenic mice were treated with the iPLA2γ inhibitor (R)-BEL. Slices were then processed for confocal immunofluorescence microscopy with an antibody recognizing Tau phosphorylation at Threonine 231 epitopes (AT231, in green) (AbCam, Cambridge, MA, USA. Dilution 1 : 750). When compared to controls (upper panel), immunostaining revealed increased phosphorylation in the CA1 region of cultured hippocampal slices incubated with 3 μM (R)-BEL for a period of 12 h (lower panel). DAPI (in blue) was included in the mounting medium to label nuclei. This observation was qualitatively reproduced in hippocampal slices obtained in 3 different cultures. Scale bar = 25 μm.
