Research Article

Hippocampal Dendritic Spines Modifications Induced by Perinatal Asphyxia

Figure 1

Micrographs of Stratum radiatum of CA1 hippocampal area from 4-month-old control rats and rats subjected to 19 min of PA. Vibratome sections of 50 μm were cut and stained with cresyl violet (Left), analysed by electron microscopy (EM) (Middle) and NeuN immunostaining (Right). A clear nuclear condensation was observed after 19 min of PA. Electron micrograph showed that most of the condensed cells correspond to neurons in degeneration. Abnormal NeuN+ nuclei were increased in asphyctic animals respect to control group. Scale bar: 30 μm and 0.5 μm for EM. Nu: nucleus.
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