Hippocampal CA1 Pyramidal Neurons of Mecp2 Mutant Mice Show a Dendritic Spine Phenotype Only in the Presymptomatic Stage
Figure 1
Dendritic spines in the CA1 region of the hippocampus visualized by DiI “DiOlistics” and confocal microscopy. (a) (left), Brightfield image of the CA1 region of a representative formalin-fixed hippocampal section (100 μm thickness) stained with DiI by particle-mediated labeling (DiOlistics) showing tungsten bullets used to deliver DiI (arrows). (right), DiI fluorescence from the same field of view. (b) Representative CA1 pyramidal neuron stained with DiI and imaged by confocal microscopy. Inset: apical dendritic segment representative of those selected for quantitative analyses of dendritic spines.
