NMDA Reduces Tau Phosphorylation in Rat Hippocampal Slices by Targeting NR2A Receptors, GSK3β, and PKC Activities

Figure 1

Tau phosphorylation at Ser199-202 sites is reduced after NMDA treatment. Phosphorylation and protein levels were estimated by Western blotting of cell extracts (40 μg proteins) obtained from hippocampal slices treated with increasing concentrations of NMDA for 1 hour. Phosphorylated Tau levels at Ser199-202, expressed relative to total Tau (Tau-5) levels, were measured in slices treated with or without NMDA. The data were expressed as percentages of control values and are means ± S.E.M. of 3 measurements per cell extract obtained from 8 different rats. For statistical analysis, one-way ANOVA was followed by Newman-Keuls post hoc test. *𝑃<0.05, **𝑃<0.01, NMDA-treated versus control.