NMDA Reduces Tau Phosphorylation in Rat Hippocampal Slices by Targeting NR2A Receptors, GSK3β, and PKC Activities
Figure 2
NMDA-induced changes in Tau phosphorylation is isoform-specific. Phosphorylated Tau levels at Ser199-202 were estimated by Western blotting of cell extracts obtained from acute hippocampal slices treated with or without 10 μM NMDA for 1 hour. Four Tau isoforms were detected in blots developed with antibody directed against Ser199-202. Expressed relative to total Tau (i.e., Tau-5) levels, the data indicate that NMDA-induced reduction of Tau phosphorylation was striking for the 62-kDa isoform and, at a lesser degree, for the 56-kDa isoform. The data are expressed as percentages of control values and are means ± S.E.M. of 3 measurements per cell extract obtained from 8 different rats. Since each isoform was investigated independently, statistical significance was determined by unpaired t-test. *, **, NMDA-treated versus respective control.
