NMDA Reduces Tau Phosphorylation in Rat Hippocampal Slices by Targeting NR2A Receptors, GSK3β, and PKC Activities
Figure 5
NMDA-induced changes in Tau phosphorylation are mediated by calcium and GSK3β. (a) Phosphorylated Tau levels at Ser199-202 were estimated by Western blotting of cell extracts obtained from hippocampal slices treated with 10 μM NMDA for 1 hour alone or in combination with 10 μM BAPTA. The data are expressed relative to total Tau (i.e., Tau-5) levels. (b) Phosphorylated GSK3β levels at Ser9 and Tyr216 epitopes were estimated by Western blotting of cell extracts obtained from hippocampal slices treated with 5 μM NMDA for 1 hour. The data are expressed relative to total GSK3β levels as percentages of control values and are means ± S.E.M. of 3 measurements per cell extract obtained from 9 different rats. For statistical analysis, one-way ANOVA was followed by Newman-Keuls post hoc test. **, ***, drug-treated versus control.
