NMDA Reduces Tau Phosphorylation in Rat Hippocampal Slices by Targeting NR2A Receptors, GSK3β, and PKC Activities
Figure 7
NMDA-induced changes in GSK3β Ser9 phosphorylation are mediated by PKC activation. Phosphorylated GSK3β levels at Ser9 and Tyr216 epitopes were estimated by Western blotting of cell extracts obtained from hippocampal slices treated with 5 μM NMDA for 1 hour alone or in combination with the PKC inhibitor Chelerythrine chloride (Chele; 1 μM). The data are expressed relative to total GSK3β levels and as percentages of control values. They are the means ± S.E.M. of 3 measurements per cell extract obtained from 7 different rats. For statistical analysis, one-way ANOVA was followed by Newman-Keuls post hoc test. ***, drug-treated versus control.
