Subthalamic hGAD65 Gene Therapy and Striatum TH Gene Transfer in a Parkinson’s Disease Rat Model
Figure 4
Quantification of tyrosine hydroxylase-positive cells in the SNc and VTA. There was no statistical difference in the number of tyrosine hydroxylase-positive cells in the SNc and VTA between the subgroups of fibroblasts (TH), rAAV2-hGAD65, and combination at 4, 8, and 16 weeks after treatment (, ). At the same time, there was no statistical difference in the number of the TH-positive neurons in these three groups and the three control groups (fibroblasts, rAAV2 vector, and PBS) (, ). There was a decreasing tendency of TH-positive neurons of SNc and VTA of all six groups from 4 weeks to 16 weeks after treatment. Fibroblasts (TH), fibroblasts transferred by rAd-TH; fibroblasts, the primary cultured fibroblasts; rAAV2-hGAD65, recombinant adeno-associated virus particles containing human glutamic acid decarboxylase 65; rAAV2 vector, control adeno-associated virus pedicles; combination, rAAV2-hGAD65 + fibroblasts (TH); PBS, inject the PBS into the STN.
