Treadmill Exercise Induces Hippocampal Astroglial Alterations in Rats

Table 1

Experimental sets of rats submitted to treadmill exercise.

Experimental set	𝑁	Biochemical assays	Samples
1	11	Corticosterone	Blood
2	20	Immunohistochemistry for GFAP; ELISA for GFAP, S100B, and BDNF; GSH and NO measurement	Hippocampal tissue
3	17	Glutamate uptake and S100B secretion in slices	Hippocampal tissue

Adult Wistar rats were submitted to 4 weeks (20 min, 5 times a week) of treadmill exercise. At 2 h (set 1) or 24 h (sets 2 and 3) after the last exercise session, rats were anesthetized, killed, and samples were collected for the biochemical assays, as described in Section 2.