Table 1: Experimental sets of rats submitted to treadmill exercise.

Experimental set Biochemical assaysSamples

111CorticosteroneBlood
220Immunohistochemistry for GFAP; ELISA for GFAP, S100B, and BDNF; GSH and NO measurementHippocampal tissue
317Glutamate uptake and S100B secretion in slicesHippocampal tissue

Adult Wistar rats were submitted to 4 weeks (20 min, 5 times a week) of treadmill exercise. At 2 h (set 1) or 24 h (sets 2 and 3) after the last exercise session, rats were anesthetized, killed, and samples were collected for the biochemical assays, as described in Section 2.