Improved Method for the Quantification of Motility in Glia and Other Morphologically Complex Cells
Figure 2
Image preprocessing for analysis. (a) Maximum intensity projections of two-photon images of a radial glia cell expressing farnesylated EGFP in vivo collected at 0, 5, and 10 minutes. (b) Same images after Sobel edge detection and binarization. (c) Binarized images underwent dilation with a 6-pixel radius. Pixel dilation helps eliminate artifactual discontinuities in the images (arrowhead in insets) resulting from the binarization step. Scale bar = 10 m.
