G-Protein Coupled Receptor-Evoked Glutamate Exocytosis from Astrocytes: Role of Prostaglandins

Figure 1

VGLUT1-pHluorin is mainly expressed on a specific population of glutamatergic synaptic like microvesicles. In the figure the left panels (in green) show astrocytes transfected with VGLUT1-pHluorin construct revealed by rabbit antibody against GFP. The middle panels (in red) show the markers of the intracellular secretory organelles, revealed by mouse antibodies against specific markers of ((a)–(e)) synaptic like microvesicles ((a) VGLUT1, (b) VGLUT2, (c) VAMP3, (d) glutamate, (e) VAMP2), of (f) dense core granules (phogrin), of (g) late endosomes, multivesicular bodies and lysosomes (LAMP1), of (h) early endosomes (EAA1), and of (i) recycling endosomes (transferrin receptor, Tfr). The right panels show the merged images. Bars: 20 μm.

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