G-Protein Coupled Receptor-Evoked Glutamate Exocytosis from Astrocytes: Role of Prostaglandins
Figure 5
Extracellular PGE2: accumulation in response to various stimuli and effects on exocytosis of glutamatergic vesicles. (a) Extracellular accumulation of PGE2 (expressed as pg/mL) in response to 3 min stimulation with either t-ACPD+ AMPA (each at 50 μM) or ATP (100 μM). Each point represents the average ± SEM of two experiments in triplicate with each stimulus. (b) Temporal distribution of fusion events evoked by PGE2 (50 μM). Inset histograms represent the total number of fusion events evoked by PGE2 () in the presence of 0 mM Ca2+ and 5 mM of EGTA () or cyclopiazonic acid (CPA, 10 μM, ). (c) Temporal distribution of fusion events evoked by DHPG (100 μM). (d) Inhibitory effect of AbPGE2 (buffering capacity >1000 pg/mL PGE2) on exocytosis of glutamatergic vesicles evoked by DHPG (100 μM). Histograms represent temporal distribution of fusion events evoked by DHPG in the presence of AbPGE2. Statistical significance was calculated using t-test (*).
