Dual Effect of Exogenous Nitric Oxide on Neuronal Excitability in Rat Substantia Gelatinosa Neurons

Figure 3

Fluorescence response of NO in DAF-FM DA-loaded spinal cord slices. (a) After addition of SNP (1 mM), fluorescence intensity increased. Hemoglobin (50 μM) (upper) and PBN (2 mM) (lower) prevented the NO-induced fluorescence increase (scale bars: 50 μm). ((b), (c)) Obtained images during the time series were shown for changes in fluorescence intensity within the regions of interest (ROI) (arrows indicate ROI). ((d), (e)) The results were quantitatively analyzed as percent units of DAF fluorescence of the control. *Values are significantly different from the control (SNP), based on independent 𝑡-test analysis (𝑃<0.05). Mean ± SEM.

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